The theory is right, but the liver really needs to be frozen through to see the results properly. The variables that affect the decomposition of hydrogen peroxide are: 1. I repeated my results so that I could get an average and get more accurate results. On the large plate, test a drop of the treated liver with hydrogen peroxide and record your results in your data table. Set up your test tube. The liver was not cut up equally so there for the larger the surface area the molecules have a bigger surface to work on. Because liver has a higher concentration of catalase than potato it means that when it is placed in hydrogen peroxide it will produce more oxygen in a set time limit.
You can ask any homework question and get expert homework help in as little as two hours. How did particle size affect the rate of reaction? A lab coat must be warn to prevent spillages on skin and clothing Safety glasses to prevent splashes into eyes Gloves to prevent contact onto skin All spillages must be reported and cleaned up immediately. The cold water does not work as well, mostly because the cells within the liver do not have time to get cold enough for this to work. This was a problem on my own because the reaction happened fast and my readings were almost certain to be inaccurate. In this lab, you will study an enzyme that is found in the cells of many living tissues.
Catalase reacts with hydrogen peroxide to form water and oxygen in the cells. Different tissues in the body have different pHs pH is a measure of how basic or acidic a solution is. Why don't cancer cells die? That is what it takes to understand how the world works, even if the experiment isn't pretty. What does the mixture look like? Works Cited What are Enzymes? The liver uses specialized enzymes to help it break down toxic substances and make them safer for the body to process. In the second part of the experiment, the intensity of the reaction in ascending order was apple, potato, and liver. Background Info- This experiment will involve some soft potato.
Which one reacted the least? For more about how we homeschool, subscribe to my or like. Although the cells are dead, catalase still remains active. After this point it doesn't matter how much more substrate you add the rate of reaction will not increase anymore as the enzymes are working as fast as they can to break down the substrate. I know this because my research shows that when there is higher concentration of catalase then there is a faster reaction rate. Reading and following the safety precautions of all materials used in a project is the sole responsibility of each individual.
The potato is a tubur, which means it is a store ott tor plan t ts. Certain catalysts work for certain kinds of reactions; in other words, each enzyme has a particular type of reaction that it can activate. This also applies to the surface area of the cells containing the catalase. What gas is being released? Substance Apple Potato Yeast Rate of Reaction 0-5 Synthesis -- Do all living tissues contain catalase? Place 2ml of the 3% hydrogen peroxide solution into a clean test tube. Surface area of the liver could have been a factor. Put a piece of liver into the bottom of a clean test tube and cover it with a small amount of water. Treatments Bubble Untreated 5 No hydrogen peroxide added 0 Heated Frozen Acidic Basic Salted Table 1.
The questions of how environmental factors affect the rate of enzyme-catalyzed reactions were answered in this lab. Explain why there is a difference in the rates of reaction between the liver and the potato Liver contains more of the enzyme catalase, which breaks down hydrogen peroxide. Heat covered on high for 20 seconds. Make sure rubber tube is connected to the bung and gas syringe. This could have also been down to human error where measurements were inaccurate. I will be studying the effect of concentration of the catalase in this reaction.
Hydrogen peroxide is a waste product produced during respiration. Which potato sample decomposed the most hydrogen peroxide? Some types of cells contain more catalase than other types of cells. Is the process faster or slower when compared to the control experiment conducted at room temperature? Hydrogen Peroxide and Liver Experiment What happens to the substance in the liver that interacts with the hydrogen peroxide when these two substances are mixed? This is because the heat degraded the catalase enzyme, making it incapable of processing the hydrogen peroxide. Place this test tube in a boiling water bath for 5 minutes. Introduction This lab covered the measuring of the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. Our Experts won't do the work for you, but they will make suggestions, offer guidance, and help you troubleshoot. Catalase speeds up the decomposition of hydrogen peroxide into water and oxygen as shown in the equation below.
You will prepare the contents as outlined below for each corresponding test tube: a. Before the actual start of the practical the 6 petri dishes should be collected and with the marker that is available should be labelled. Certain things affect the reaction rate of a substance where an enzyme is used. Once this was done, we took the leftover liquids and used it on a new piece of meat to see if the enzyme was reusable. The speed at which this modification occurs relies on two main factors; the amount of collisions between reacting particles and what portion of these collisions are successful in producing a change within the substances present. Unless you can freeze the liver for hours, it won't really work.
An adult may need to help with this. What is the reaction rate for the boiled liver and peroxide? Procedure a Measure 25 cm 3 of hydrogen peroxide solution into each of three conical flasks. Basic solutions slow enzymatic reactions because they don't allow a reaction to properly occur. A toxin like hydrogen peroxide must be broken down because if it is kept in the body for too long it can react with cell walls and damage them or break them down. A glowing splint of a match was lit, blown out then inserted into the test tube. Pour off the liquid into a second test tube. Then increase the surface area by cutting the cubes even smaller, keeping the volume the same in each but changing the surface area.