Part B - What Tissues Contain Catalase? The reaction between hydrogen peroxide and catalase is as follows: 2H. Where Does Hydrogen Peroxide come from in the Body? One 10-ml graduated cylinder to measure the hydrogen peroxide and 40 ml of a 3% hydrogen peroxide solution. I'm doing biology online and I just watched a video showing the reactions of liver in water when hydrogen peroxide is added and I'm sort of confused about the results. What does the mixture look like? Part C: If the liver is placed in 2 ml of hydrogen peroxide at room temperature, then there will be a high rate of reaction, otherwise the enzyme will become denatured at too high or too low a temperature. In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things.
In what ways does this show a chemical reaction is occurring? It kills bacteria and the bubbling of the escaping oxygen helps to clean out dirt and bacteria from the wound. Is the liver and the potato part of the reaction? Heat is much more dramatic, and I stopped even trying with the cold reaction. The name catalase for the enzyme present in all these foodstuffs can be introduced. Why do we need catalase in our liver? The hydrogen peroxide is broken down, so that it cannot be dangerous anymore, into water which is given off when you perspire and into oxygen which can be given off when you exhale. They answer basic questions, like how do fireflies create light? Some enzymes can be damaged under certain conditions, such as heat. Introduction A chemical reaction is when chemicals come together and their molecules interact to form new chemicals.
The theory that catalase can be destroyed was tested in a lab setting using the procedures provided by Mrs. You are observing catalase breaking hydrogen peroxide into oxygen and water. The optimal ph for catalase is neutral, because that it what most of the body is comprised of. In this science fair project, you will use fresh or frozen liver as a source of catalase and investigate how the activity of the enzyme can change under certain conditions. A glowing splint of a match was lit, blown out then inserted into the test tube.
Our extensive online study community is made up of college and high school students, teachers, professors, parents and subject enthusiasts who contribute to our vast collection of study resources: textbook solutions, study guides, practice tests, practice problems, lecture notes, equation sheets and more. Beyond basic research, biologists might also apply their research and create new biotechnology. When added to the liver, hydrogen peroxide activates the renal enzyme catalase. This article is distributed under the terms of an Attribution—Noncommercial—Share Alike—No Mirror Sites license for the first six months after the publication date see. This is because the substrate, the peroxide, had been totally broken down and all that was left behind was simple water, which does not react with the enzyme. This collection of over 200 practical activities demonstrates a wide range of chemical concepts and processes. Without them the reactions would be so slow that life would grind to a halt.
Pepsin is found in the stomach, amylase in the saliva, and phosphatase in the liver. Using forceps and scissors cut a small piece of liver and add it to the test tube. Catalase decomposes, or breaks down, hydrogen peroxide into water and oxygen. To each of the three tubes add 2 ml of hydrogen peroxide. In this lab, we observed a normal catalase reaction, what tissues contain catalase, and what is the effect of temperature on catalase activity.
The liver did not float nor did it turn white. Apparatus Trough, bung, water, burette, hydrogen peroxide, liver 1. In order to obtain energy and building blocks from food, the digestive system must break down proteins, fats, and carbohydrates. The catalase found in chicken liver did react. Enzymes lower the activation energy, which speeds up reactions.
The pH of the hydrogen peroxide 4. Similarly their nature as large protein molecules whose catalytic activity can be very specific to certain chemical reactions may be unfamiliar. Light can also break down H 2O 2 which is why the chemical is sold in dark containers. If students have not performed the glowing splint test for oxygen for some time, they may need reminding of how to do so by a quick demonstration by the teacher. Wouldn't the chilled water have illicited little to no reaction at all, because the catalase works best around body temperature and a bit higher 37 - 40 degrees celsius? The optimal temperature of catalse is 98. On the large plate, test a drop of the treated liver with hydrogen peroxide and record your results in your data table. This may seem like a stupid question, but based on all I've read online about catalase and optimal temperature, I don't understand why the cold water bubbled and frothed so much when the boiled water barely reacted.
A thermometer and a stirring rod. Therefore my hypothesis is that the rate of decomposition will be at its fastest with a temperature of forty degrees Celsius and a surface area of grinded liver. For any other use, please contact Science Buddies. These reactions are usually very fast and spontaneous, and give off energy. Then cut a small piece of potato and add it to the solution and record what happens. Part A First, place 2 ml of the 3% hydrogen peroxide solution into a clean test tube. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances--water and oxygen.
Data Analysis The reaction rate of catalase on hydrogen peroxide was measured on a scale of 0-5 0 being no reaction and 5 being fastest rate based off the observation of bubbles during the reaction. A toxin like hydrogen peroxide must be broken down because if it is kept in the body for too long it can react with cell walls and damage them or break them down. Hydrogen peroxide is produced to kill off dangerous bacteria. The following graph shows reaction rates of various enzymes in the body. The bubbling reaction you see is the metabolic process of decomposition, described earlier. Fresh cow liver, chicken, apples an d potatoes.