Lab Manual for General Microbiology. The tests selected were based on the results of a gram stain. We gained knowledge and experience in both chemistry and teamwork. Gram staining indicates if there is peptidoglycan in the cell walls. To determine the amount of water found in a hydrate experimentally, you must remove the water from the hydrate.
Weight of dish and dry salt: 50. Discuss the validity and reliability of your data in answering the question. Determination of Chloride in an Unknown Salt By Diane Krehbiel Abstract: In this experiment, the chloride content of an unknown salt will be determined using two different methods. For example, a nutrient agar stock plate for B. This study was conducted using techniques and experiments learned in microbiology lab that were used to classify the two unknown bacteria. It can cause many illnesses, from minor skin infections, to life threatening diseases such as meningitis, pneumonia, osteomyelitis, endocarditis, toxic shock syndrome, and sepsis Todar, 2012.
Several biochemical tests were chosen based on the identification table which was given by the lab instructor. After a Gelatin and a Galactose test were performed, the only bacterium that remained was Pseudomonas aeruginosa. This results in complete clearing of the blood around the colonies. A Catalase test was the performed for clearification. A positive catalase test verified that the bacteria in the unknown would have to be S.
The precipitate formed will adsorb its own ions to form an adsorption layer. Twenty percent of the human population are carriers of S aureus, which can be found on the skin and inside the nasal passages. However, if no bubbles are observed, the culture is negative for catalase. Habituation of Exploratory Behavior in Rats Regarding Spatial Rearrangement of Familiar Objects in an Open Field Habituation of Exploratory Behavior in Rats Regarding Spatial Rearrangement of Familiar Objects in an Open Field Habituation is Although it might seem obvious that rats would habituate to an unfamiliar environment after repeated exposure, it is not as obvious how behavior would change as a result of new stimuli within that familiar environment. After a second round of tests, all of the results matched up with the expected results of P. Weight of dish and solution: 53. The process of identification was achieved by utilizing procedures learnt during the semester.
Had the hot plate been working properly, we would have obtained a better amount of salt solution to put in the evaporating dish. Lactose and sucrose sugar fermentation were tested using a broth containing the respective sugar compound, phenol red, and inverted Durham tubes. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. No difficult problems occurred with the identification of B. We are interested in the renewal of exploration following rearrangement of objects within the open field apparatus used as the initial novel surroundings for the rats. The solution turned cloudy white.
The methods that were previously studied and practiced in the Microbiology laboratory class were applied in order to identity two unknown bacterium. If no color changes during the flame test then place an adequate amount of salt x in a test tube and prepare a solution of it by adding water. If the sample is not dry, put in oven for at least 2 hours at 120°. If I perform theses experiments correctly and accurately then I will be able to identify my unknown bacteria. The writing of laboratory reports is an essential part of any practical module in Psychology.
To begin, prepare and standardize 0. This experiment is also used to compare the two methods used to discover chloride content in a salt. Get a 125 mL Erlenmeyer flask, add in the unknown salt sample and 40 mL of distilled water. We ended up to work with the little solution that we had. After the Gram stain was determined, specific biochemical tests were performed.
From that, the plates were incubated for two days and the bacterium grew. Another person that did the same experiment as us had 49. Weigh the dish and salt solution. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the microbiology laboratory class. A positive citrate test ruled out P. This report must be typed and any diagrams, figures, or tables should be embedded into the document.
Then, the bisulfite solution 1 ml was also added, and then the unknown 5-10 drops was added. Get a 400 mL beaker and fill it up halfway with water. When a rat becomes more familiar with its environment, exploratory behavior decreases as a function of time spent within the new area. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. Introduction: Blue Baby Syndrome is a condition that affects many infants.
If the same force is applied to an object with a. The iron agar slant was used to test for the fermentation of glucose and lactose, as well as the production of H2S. Be sure to use the same balance when weighing each time. Various tests were conducted on each the unknown bacterial cultures. Neither you, nor the coeditors you shared it with will be able to recover it again. After filtering off the solution, all that will be left is the precipitate on the filter paper. This same method can be seen in medical studies for new drugs.